Journal
/
Biology
/
Kvantitative lokalisering af Golgi Protein af Imaging dens centrum af fluorescens masse
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Kvantitative lokalisering af Golgi Protein af Imaging dens centrum af fluorescens masse

DOI:
10.3791/55996-v

•

13:08 min

•

August 10, 2017

•

, , , ,
1School of Biological Sciences,Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing,National University of Singapore

Chapters

  • 00:05Title
  • 00:35Cell Seeding
  • 02:00Cell Transfection and Nocodazole Treatment
  • 02:50Image Acquisition of Multi-color Beads and Golgi Mini-stacks
  • 04:36Image Analysis, Acquire Centers of Fluorescent Beads
  • 07:57Select and Measure Golgi Mini-stacks
  • 10:59Results: Imaging the Golgi’s Center of Fluorescence Mass
  • 12:31Conclusion

Summary

Automatic Translation

Automatic Translation

Den præcise lokalisering af Golgi beboere er afgørende for forståelsen af Golgi cellulære funktioner. Men konventionelle Optisk mikroskopi er afskåret fra løse sub-Golgi strukturen. Her beskriver vi protokol for en konventionel mikroskopi baseret super-resolution metode til at bestemme kvantitativt sub-Golgi lokalisering af et protein.

Tags

Keywords: Quantitative LocalizationGolgi ProteinFluorescence MassHeLa CellsTransfectionNocodazoleFluorescent BeadsImagingMicroscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55996?article_type=v

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
check_url/55996?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/55996?article_type=v

Analyzing Large Protein Complexes by Structural Mass Spectrometry
check_url/55996?article_type=v

En protokol til identifikation af protein-protein interaktioner Udfra<sup> 15</sup> N metabolisk mærkning Immunoprecipitation, Kvantitativ massespektrometri og Affinity Modulation
check_url/55996?article_type=v

Kvantitativ analyse af Autophagy hjælp Advanced 3D fluorescensmikroskopi
check_url/55996?article_type=v

Flow cytometri af bimolekylær Fluorescens Complementation: En High Throughput Kvantitativ metode til at studere protein-protein interaktion
check_url/55996?article_type=v

Samtidig Multicolor Imaging af biologiske strukturer med Fluorescence Fotoaktivering Lokalisering Microscopy
check_url/55996?article_type=v

Hurtig analyse og udforskningen af ​​fluorescensmikroskopi Images
check_url/55996?article_type=v

Karakterisering af G-protein-koblede receptorer med en fluorescens-baserede calciummobilisering Assay
check_url/55996?article_type=v

In Vivo 4-Dimensional sporing af hæmatopoietisk stamcelletransplantation og stamcelle i Voksen Mouse calvarie knoglemarv

Read Article