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Transplantation af Schwann celler inde PVDF-TrFE ledningsnet til bro Transected rotte rygmarven træstubbe for at fremme Axon regenerering over kløften
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JoVE Journal Medicine
Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap

Transplantation af Schwann celler inde PVDF-TrFE ledningsnet til bro Transected rotte rygmarven træstubbe for at fremme Axon regenerering over kløften

DOI:
10.3791/56077-v

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08:05 min

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November 03, 2017

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, , ,
1The Miami Project to Cure Paralysis,University of Miami Miller School of Medicine, 2Department of Materials Science and Engineering,New Jersey Institute of Technology, 3Department of Biomedical Engineering,New Jersey Institute of Technology, 4Department of Cell Biology,University of Miami Miller School of Medicine, 5Department of Neurological Surgery,University of Miami Miller School of Medicine

Chapters

  • 00:05Title
  • 01:19Complete Transection at Thoracic Level 8 (T8)
  • 03:47Transection at T8, Conduit Insertion, and Schwann Cell/Matrix Injection
  • 06:36Results: Representative Transplanted Spinal Cord Section Images
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

I denne artikel beskrives en teknik til at indsætte en hule conduit mellem rygmarven stubbe efter komplet transection og fyld med Schwann celler (SCs) og injicerbar basalmembranen matrix for at bygge bro og fremme axon regeneration på tværs af hullet.

Tags

Schwann CellsPVDF-TrFE ConduitsSpinal Cord TransectionAxon RegenerationLaminectomyRat ModelSurgical Procedure

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