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Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
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JoVE Journal Medicine
Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

DOI:
10.3791/56084-v

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06:14 min

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October 15, 2017

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, , ,
1Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences,Monash University, 2Monash Biomedicine Discovery Institute, Department of Microbiology,Monash University

Chapters

  • 00:00Title
  • 00:18Introduction
  • 04:11Results
  • 05:05Conclusion

Summary

Automatic Translation

Automatic Translation

Ivacaftor and ivacaftor-lumacaftor combination are two new CF drugs. However, there is still a dearth of understanding on their PK/PD and pharmacology. We present an optimized HPLC-MS technique for the simultaneous analysis of ivacaftor and its major metabolites, and lumacaftor.

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Keywords: LC-MS/MSIvacaftorMetabolitesLumacaftorCystic FibrosisPlasma AssaySample PreparationStandard PreparationInternal StandardProtein PrecipitationCentrifugationFiltration

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