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全睾丸针浸泡超快速冷却法超低温保存斑马鱼细胞

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Biology
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JoVE Journal Biology

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Please note that all translations are automatically generated. Click here for the English version.

全睾丸针浸泡超快速冷却法超低温保存斑马鱼细胞

DOI:

10.3791/56118-v

•

13:32 min

•

March 04, 2018

•

Zoran Marinović, Jelena Lujić, Eszter Kása, Zsolt Csenki, Béla Urbányi, Ákos Horváth

¹Department of Aquaculture,Szent István University

Chapters

00:00Title
01:14Testis collection
03:29Ultra-rapid cooling of the testicular tissue
06:11Warming procedure
07:56Tissue digestion
10:27Viability evaluation and cell counting
11:28Representative Results
12:20Conclusions

Summary

Automatic Translation

本研究的主要目的是适应针浸泡玻璃化 (cryopreserve) 程序, 以使整个斑马鱼睾丸。另外, 对五种不同斑马鱼菌株的重复性进行了测试。

Tags

Keywords: Cryopreservation Zebrafish Spermatogonia Vitrification Whole Testes Ultra-rapid Cooling Germ Cells Transplantation Slow-rate Freezing Spermatozoa Euthanasia Dissection Testes Removal Sterilization Equilibration Vitrification Solutions

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