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Kryopræservering af zebrafisk Spermatogonia af hele testiklerne nål nedsænket ultra-hurtig nedkøling
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Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Kryopræservering af zebrafisk Spermatogonia af hele testiklerne nål nedsænket ultra-hurtig nedkøling

DOI:
10.3791/56118-v

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13:32 min

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March 04, 2018

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, , , , ,
1Department of Aquaculture,Szent István University

Chapters

  • 00:00Title
  • 01:14Testis collection
  • 03:29Ultra-rapid cooling of the testicular tissue
  • 06:11Warming procedure
  • 07:56Tissue digestion
  • 10:27Viability evaluation and cell counting
  • 11:28Representative Results
  • 12:20Conclusions

Summary

Automatic Translation

Automatic Translation

Det vigtigste formål med denne undersøgelse var at tilpasse nålen nedsænket vitrifikation (NIV) proceduren for at cryopreserve hele zebrafisk testiklerne. Derudover blev repeterbarhed af metoden i fem forskellige zebrafisk stammer testet.

Tags

Keywords: CryopreservationZebrafishSpermatogoniaVitrificationWhole TestesUltra-rapid CoolingGerm CellsTransplantationSlow-rate FreezingSpermatozoaEuthanasiaDissectionTestes RemovalSterilizationEquilibrationVitrification Solutions

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