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Stereotactic Atlas-styrede Laser fange Microdissection af hjerneregioner ramt af traumatisk skade
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
DOI:

09:29 min

September 11, 2017

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Chapters

  • 00:05Title
  • 00:59Region Identification and Sectioning
  • 04:01Staining Protocol
  • 04:50Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System
  • 07:36Results: LCM of Traumatic Brain Injury affected Brain Regions
  • 08:55Conclusion

Summary

Automatic Translation

Vi beskriver brugen af laser fange microdissection at få prøver af forskellige cellepopulationer fra forskellige hjerneregioner for gen og mikroRNA analyse. Denne teknik giver mulighed for undersøgelse af differentiale virkninger af traumatisk hjerneskade i specifikke områder af hjernen, rotte.

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