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Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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Developmental Biology
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JoVE Journal Developmental Biology
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

DOI:
10.3791/56146-v

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10:48 min

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July 07, 2017

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, , , , ,
1Department of Neuroscience,Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

Chapters

  • 00:05Title
  • 01:08Biopsy Handling
  • 03:42Sendai Virus Vector Reprogramming of Fibroblasts
  • 05:17Picking of Colonies and Expansion of hiPS Cells
  • 08:21Results: Successful Generation of Defined and Xeno-free hiPS Cells from Fiboblasts
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

Tags

Keywords: Human Induced Pluripotent Stem CellsIntegration-free DerivationLaminin 521 MatrixSkin BiopsyDispaseCollagenase IXeno-freeChemically Defined Culture SystemDisease ModelingDrug ScreeningCell Therapy

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