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Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility
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JoVE Journal Neuroscience
Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility

Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility

DOI:
10.3791/56147-v

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07:49 min

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July 27, 2017

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, , ,
1Division of Biological Sciences,University of California San Diego

Chapters

  • 00:05Title
  • 00:50Odor Delivery Cartridge for Type IV Antennal Trichoid Sensillum (AT4) Recording
  • 01:58Preparing Drosophila for Electrophysiological Recordings while Stimulating AT4
  • 03:46Taking Electrophysiology Recording of AT4 Sensillum Activity
  • 06:06Results: Isometric Sensitivity of Or47b Neurons to Palmitoleic Acid
  • 07:06Conclusion

Summary

Automatic Translation

Automatic Translation

The overall goal of this protocol is to demonstrate how to present odorants of low volatility for single-sensillum recording from Drosophila olfactory receptor neurons that respond to long-chain cuticular pheromones.

Tags

Electrophysiological RecordingDrosophila Trichoid SensillaOdorantsLow VolatilityOlfactory Receptor NeuronsPheromone CommunicationOdor DeliveryPipette TipFilter PaperFly PreparationAspirator TubeMolding Clay

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