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JoVE Journal Cancer Research
Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
 

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Article DOI: 10.3791/56159-v • 08:57 min • October 5th, 2017
October 5th, 2017 •
1, 1, 1, 1, 1, 2, 1, 1
1Department of Pharmaceutical Sciences, University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

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Summary
Automatic Translation

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

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Keywords: Doxorubicin Mitomycin C Doxorubicinol Nanoparticles Tumor HPLC Simultaneous Quantitation Sample Preparation Tissue Homogenization Extraction Internal Standard
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