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Optisk tværsnit muskel område bestemmelse af Drosophila Melanogaster voksen indirekte flyvning muskler
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JoVE Journal Biology
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Optisk tværsnit muskel område bestemmelse af Drosophila Melanogaster voksen indirekte flyvning muskler

DOI:
10.3791/56179-v

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06:43 min

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March 31, 2018

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, ,
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED),University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

Chapters

  • 00:00Title
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

Automatic Translation

Automatic Translation

Vi rapporterer en metode til at kvantificere muskel område, som er en indirekte metode til at bestemme muskelmasse i Drosophila voksne. Vi demonstrere anvendelsen af vores metodik ved at analysere den indirekte flyvning musklerne i Drosophila model af født dystrofi sygdom.

Tags

Drosophila MelanogasterOptical Cross-sectional Muscle AreaIndirect Flight MusclesMuscle MassMuscle AtrophyDorsal Longitudinal MusclesFixationResin EmbeddingAnesthesiaDehydrationPropylene OxideResin PolymerizationTrimmingSectioningUltramicrotome

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