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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Genetics
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JoVE Journal Genetics
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

DOI:
10.3791/56187-v

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11:42 min

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October 27, 2017

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, ,
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Chapters

  • 00:05Title
  • 01:04Generating Embryos Through Natural Mating and Staging Embryos
  • 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
  • 04:55Sorting Differentially Expressed Genes
  • 07:00Determine Enriched Pathways
  • 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
  • 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
  • 10:47Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.

Tags

Keywords: RNASeqGene ExpressionZebrafishEmbryoLarvaTranscriptomeSample PreparationRNA ExtractionRNA SequencingDevelopmental BiologyComparative Analysis

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