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Eksempel forberedelse og analyse av RNASeq-baserte genuttrykk Data fra sebrafisk
JoVE Journal
Genetics
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JoVE Journal Genetics
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
DOI:

11:42 min

October 27, 2017

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Chapters

  • 00:05Title
  • 01:04Generating Embryos Through Natural Mating and Staging Embryos
  • 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
  • 04:55Sorting Differentially Expressed Genes
  • 07:00Determine Enriched Pathways
  • 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
  • 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
  • 10:47Conclusion

Summary

Automatic Translation

Denne protokollen gir en tilnærming for hele transcriptome analyse fra sebrafisk embryo, larvene, eller sortert celler. Vi inkluderer isolering av RNA, sti analyse av RNASeq data, og qRT-PCR-baserte valideringen av genet uttrykk endringer.

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