Journal
/
Immunology and Infection
/
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

DOI:
10.3791/56191-v

•

06:08 min

•

August 09, 2017

•

, , ,
1UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:05Title
  • 00:47Effector Cell and K562 Target Cell Preparation
  • 02:24Effector Cell and K562 Target Cell Co-culture ad Viability Staining and Acquisition
  • 04:23Results: Representative Human NK Cell Cytotoxixity Assessment
  • 05:20Conclusion

Summary

Automatic Translation

Automatic Translation

A flow cytometry-based method to quantitatively determine the cytotoxic activity of human natural killer cells is shown here.

Tags

Flow CytometryCytotoxicity AssayNK Cell ActivityNK Cell DeficiencyPBMCK562 CellsCFSEEffector-to-target Cell Ratio

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56191?article_type=v

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
check_url/56191?article_type=v

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
check_url/56191?article_type=v

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
check_url/56191?article_type=v

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
check_url/56191?article_type=v

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
check_url/56191?article_type=v

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
check_url/56191?article_type=v

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
check_url/56191?article_type=v

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
check_url/56191?article_type=v

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
check_url/56191?article_type=v

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Read Article