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Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
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Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

DOI:
10.3791/56199-v

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07:35 min

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May 08, 2018

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, , ,
1Department of Ophthalmology,University Medical Center of the Johannes Gutenberg University Mainz

Chapters

  • 00:05Title
  • 01:05Mouse Retina Isolation
  • 03:00Perfusion Chamber Retina Mounting
  • 04:31Retinal Arteriole Preparation and Experimentation
  • 05:58Results: Representative Functional Analyses
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.

Tags

Keywords: Retinal ArteriolesRetina PreparationMouse RetinaRetinal Blood FlowVideo MicroscopyRetinal Blood Vessel ReactivityOphthalmic ArteryKrebs-Henseleit BufferVannas ScissorsPrecision TweezersCorneaChoroidSclera

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