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游离的小鼠皮层神经元文化的制备
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JoVE Journal Biology
Preparation of Dissociated Mouse Cortical Neuron Cultures
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游离的小鼠皮层神经元文化的制备

DOI:
10.3791/562-v

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18:02 min

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December 19, 2007

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1Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:06Tools and Reagents
  • 03:13Mounting Brain onto Vibraslicer
  • 04:26Removing and Sectioning Brain
  • 08:12Isolating and Processing Cortex
  • 11:29Washing Cortical Pieces
  • 13:14Trituration of Cortical Pieces
  • 16:35Visualizing Cortical Neurons
  • 17:12Conclusion

Summary

Automatic Translation

Automatic Translation

这个视频显示了从胚胎后期和产后早期小鼠皮层神经元的文化生成的过程。这些文化可用于免疫细胞化学，生物化学，电生理，钙和钠成像，并提供一个平台，携带产后的致死基因突变的转基因动物研究神经元发育。

Tags

Dissociated Mouse Cortical Neuron CulturesLate EmbryoEarly PostnatalMouse BrainImmunocytochemistryBiochemistryElectrophysiologyCalcium ImagingSodium ImagingProtein IsolationRNA IsolationTransgenic AnimalsGene MutationCortical RindThalamo-cortical Fiber TractNon-neuronal CellsNeuronal CellsEnzyme Incubation StepGlia Feeder Cells

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