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游离的小鼠皮层神经元文化的制备
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Dissociated Mouse Cortical Neuron Cultures
Please note that all translations are automatically generated.
Click here for the English version.
游离的小鼠皮层神经元文化的制备
DOI:
10.3791/562-v
•
18:02 min
•
December 19, 2007
•
Lutz G. W. Hilgenberg
,
Martin A. Smith
1
Department of Anatomy and Neurobiology
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:15
Introduction
01:06
Tools and Reagents
03:13
Mounting Brain onto Vibraslicer
04:26
Removing and Sectioning Brain
08:12
Isolating and Processing Cortex
11:29
Washing Cortical Pieces
13:14
Trituration of Cortical Pieces
16:35
Visualizing Cortical Neurons
17:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频显示了从胚胎后期和产后早期小鼠皮层神经元的文化生成的过程。这些文化可用于免疫细胞化学,生物化学,电生理,钙和钠成像,并提供一个平台,携带产后的致死基因突变的转基因动物研究神经元发育。
Tags
Dissociated Mouse Cortical Neuron Cultures
Late Embryo
Early Postnatal
Mouse Brain
Immunocytochemistry
Biochemistry
Electrophysiology
Calcium Imaging
Sodium Imaging
Protein Isolation
RNA Isolation
Transgenic Animals
Gene Mutation
Cortical Rind
Thalamo-cortical Fiber Tract
Non-neuronal Cells
Neuronal Cells
Enzyme Incubation Step
Glia Feeder Cells
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