A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Biological Chemistry, School of Medicine,University of California, Irvine, 2Beckman Laser Institute and Medical Clinic,University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,University of California, Irvine, 4Department of Biomedical Engineering and Surgery,University of California, Irvine
Chapters
- 00:05Title
- 01:25Transient Transfection
- 04:09Titration of Laser Input Power
- 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
- 06:54Cell Cycle and G1 Phase Synchronization
- 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
- 09:27Conclusion
L'obiettivo del presente protocollo è quello di descrivere come utilizzare laser microirradiation per indurre diversi tipi di danno del DNA, compreso le rotture del filo relativamente semplici e danno complesso, per studiare assieme di fattore DNA danni segnalazione e riparazione presso siti di danno in vivo .
