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1Department of Biological Chemistry, School of Medicine,University of California, Irvine, 2Beckman Laser Institute and Medical Clinic,University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,University of California, Irvine, 4Department of Biomedical Engineering and Surgery,University of California, Irvine
Chapters
- 00:05Title
- 01:25Transient Transfection
- 04:09Titration of Laser Input Power
- 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
- 06:54Cell Cycle and G1 Phase Synchronization
- 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
- 09:27Conclusion
O objetivo do presente protocolo é descrever como usar o microirradiation do laser para induzir diferentes tipos de danos no DNA, incluindo relativamente simples quebras e danos complexos, para estudar o DNA danos reparação e sinalização fator montagem em locais de dano in vivo .
