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1Department of Biological Chemistry, School of Medicine,University of California, Irvine, 2Beckman Laser Institute and Medical Clinic,University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,University of California, Irvine, 4Department of Biomedical Engineering and Surgery,University of California, Irvine
Chapters
- 00:05Title
- 01:25Transient Transfection
- 04:09Titration of Laser Input Power
- 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
- 06:54Cell Cycle and G1 Phase Synchronization
- 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
- 09:27Conclusion
El objetivo de este protocolo es describir cómo utilizar microirradiation de láser para inducir a diferentes tipos de daños en el ADN, como roturas de cadena relativamente simple y complejo daño, para el estudio de ADN daños reparación y señalización de factor Asamblea en sitios de daño en vivo .
