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1Department of Clinical Research,University of Bern, 2Graduate School for Cellular and Biomedical Sciences,University of Bern, 3Immunology Research Centre,St. Vincent’s Hospital Melbourne, 4Institute of Pathology,University of Bern, 5Institute of Molecular Animal Breeding and Biotechnology,Ludwig-Maximilian University
Chapters
- 00:05Title
- 00:48Coating Microcarrier Beads
- 01:39Collecting Cells
- 02:26Seeding Cells into the Stirrer Flask
- 03:45Experimental Procedure
- 04:32Incubation with Non-anticoagulated Blood
- 05:46Results: Clotting
- 06:28Conclusion
हम एक इन विट्रो मॉडल जो अध्ययन और पूरे, गैर anticoagulated रक्त में जमावट के विश्लेषण की अनुमति देता है पेश करते हैं । प्रणाली में एंटिकोगुलेशन स्वस्थ endothelial कोशिकाओं के प्राकृतिक एंटिकोगुलेशन प्रभाव पर निर्भर करता है और endothelial कोशिका सक्रियण के थक्के में परिणाम होगा ।
Tags
3D Cell CultureEndothelial CellsAnticoagulant PropertiesAnti-inflammatory PropertiesWhole BloodNon-anticoagulated BloodCardiovascular ResearchTransportation ResearchSurgery ResearchAnti-immunityEndothelial Cell ActivationMicrocarrier BeadsCoagulationPhosphate Buffered Saline (PBS)Dulbecco’s Modified Eagle’s Medium (DMEM)Medium 199TrypsinEDTACell Culture MediaMagnetic Stirrer Flask
