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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
DOI:

10:44 min

November 13, 2017

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Chapters

  • 00:05Title
  • 00:51Single-transfer Slide Preparation
  • 03:24Double-transfer Slide Preparation
  • 05:53Multiplexed Immunoassays with a Snap Apparatus
  • 08:53Results: Snap Transfer of Arrays for a Multiplex Immunoassay
  • 09:59Conclusion

Summary

Automatic Translation

We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.

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