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Regenerering af klædt guld Microelectrodes udstyret til en Real-Time celle Analyzer
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1School of Public Health,Nanjing Medical University, 2School of Pharmacy,Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering,Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research,The Affiliated Cancer Hospital of Nanjing Medical University
Chapters
- 00:04Title
- 00:52Proliferation/Cytotoxicity Experiment and Data Acquisition
- 03:54Case 1: Regeneration of Electronic Plates after Cytotoxicity Evaluation of Anti-cancer Drug
- 05:53Case 2: Regeneration of Electronic Plates after Drug Delivery using Mesoporous Material
- 07:34Assessment of Regeneration Effect
- 10:22Results: Evaluationof Regenerated Electronic Plates
- 12:01Conclusion
Denne protokol beskriver en generel strategi for at regenerere kommercielle klædt guld microelectrodes udstyret til en etiket-gratis celle analyzer sigter besparelse på de høje løbende omkostninger ofmicrochip-baserede assays. Regenereringsprocessen omfatter trypsin fordøjelse, skylning med ethanol og vand og en spinning skridt, som giver mulighed for gentagen brug af mikrochips.
