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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
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JoVE Journal Chemistry
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

DOI:
10.3791/56253-v

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08:05 min

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September 29, 2017

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, , ,
1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry,Tsinghua University, 2Institute of Chemical Materials,China Academy of Engineering Physics

Chapters

  • 00:05Title
  • 00:59Synthesis of DF PEG for Hydrogels
  • 03:00Preparing Chitosan-based Hydrogels with Encapsulated Cells
  • 05:15Cell Viability Analysis
  • 06:08Results: Affect of Injection on Viability of Hydrogel-encapsulated L929 Mouse Fibroblasts
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented.

Tags

Keywords: ChitosanInjectable Hydrogel3D Cell CultureSelf-healing HydrogelDF PEGBenzaldehyde-terminated DF PEGCell EncapsulationCell Culture MediaCell Therapy Carrier

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