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استهداف ثيولس سيستين في المختبر جليكوسيليشن الخاصة بالموقع من البروتينات المؤتلف
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Chapters
- 00:05Title
- 01:09Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis
- 03:44Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
- 05:24Purification of Recombinant Protein from E. coli
- 07:50Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
- 08:48Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
- 10:11Conclusion
