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Journal / Medicine / Oral Biofilm Sampling for Microbiome Analysis in…
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JoVE Journal Medicine
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
 
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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Article DOI: 10.3791/56320-v • 10:42 min • December 31st, 2017
December 31st, 2017 •
1, 2, 1
1Division of Oral Surgery and Orthodontics, Department of Dental Medicine and Oral Health, Medical University of Graz, 2Division of Preventive and Operative Dentistry, Periodontology, Prosthodontics and Restorative Dentistry, Department of Dental Medicine and Oral Health, Medical University of Graz

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Changes in the oral microbiome throughout childhood are of growing interest. Comparison of different microbiome studies reveals a lack of standardized sampling protocols. Limited space makes sampling the sound subgingival sulcus of children challenging. Paper point sampling is presented here in detail as the method of choice for this area.

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Keywords: Oral Biofilm Microbiome Analysis Healthy Children Standardized Sampling Method Subgingival Sulcus Vestibular Fold Saliva Sterile Instruments Paper Point Sampling Plaque Disclosure Electric Toothbrush Cotton Swabs Junctional Epithelium
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