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Langsigtet Live Imaging enhed for forbedret eksperimentelle manipulering af zebrafisk larver
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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Morgridge Institute for Research,University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program,University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology,University of Wisconsin-Madison, 6Department of Pediatrics,University of Wisconsin-Madison
Chapters
- 00:05Title
- 00:57Design and 3D Printing of Molds
- 02:57PDMS Fabrication of zWEDGI Device
- 04:43Plasma-bonding zWEDGI to Glass Dish
- 07:19Channel Preparation and Loading Larvae
- 09:52Wounding and Imaging Larvae and End of Experiment
- 11:14Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
- 12:36Conclusion
Dette manuskript beskriver zWEDGI (zebrafisk Wounding og Entrapment enhed for vækst og Imaging), som er en opdelte enhed designet til at orientere og begrænse zebrafisk larver. Design tillader hale transection og langsigtede samling af høj opløsning fluorescerende mikroskopi billeder af sårheling og regenerering.
