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Journal / Bioengineering / Proper Positioning and Restraint of a Rat Hind…
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Bioengineering

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JoVE Journal Bioengineering
Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography
 

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Article DOI: 10.3791/56346-v • 04:24 min • November 22nd, 2017
November 22nd, 2017 •
1, 1, 1,2
1Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 2Department of Health Sciences, Faculty of Applied Health Sciences, Brock University

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Summary
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This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture.

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Keywords: Rat Hind Limb Bone Micro-architecture In Vivo Micro-computed Tomography Positioning Restraint Foam Tube Dental Wax Masking Tape Vet Wrap Longitudinal Studies Quantification
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