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基于状的不均匀 Nanopatterns 对局部控制表面粘附的研究--一种直接软骨分化的方法
JoVE Journal
Bioengineering
Author Produced
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JoVE Journal Bioengineering
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
DOI:

14:46 min

January 20, 2018

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Chapters

  • 00:00Title
  • 01:12Production of Nanopatterned Growth Substrates
  • 05:59Local RGD Surface Density Characterization with AFM
  • 07:49Cell Culture and Induction of Chondrogenesis
  • 08:49Cell Fixation and Immunostaining
  • 10:18Cell Imaging and Data Analysis
  • 12:28Representative Results
  • 13:29Conclusion

Summary

Automatic Translation

本文介绍了一种获得基于状的不均匀 nanopatterns 的方法, 该法允许对局部精氨酸-甘氨酸 (RGD) 的表面密度进行纳米控制, 并将其用于细胞黏附和软骨分化的研究。

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