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Bioengineering
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基于状的不均匀 Nanopatterns 对局部控制表面粘附的研究--一种直接软骨分化的方法
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
Please note that all translations are automatically generated.
Click here for the English version.
基于状的不均匀 Nanopatterns 对局部控制表面粘附的研究--一种直接软骨分化的方法
DOI:
10.3791/56347-v
•
14:46 min
•
January 20, 2018
•
Ignasi Casanellas
2
,
Anna Lagunas
1
,
Iro Tsintzou
,
Yolanda Vida
5
,
Daniel Collado
5
,
Ezequiel Pérez-Inestrosa
5
,
Cristina Rodríguez-Pereira
,
Joana Magalhaes
6
,
Pau Gorostiza
3,7
,
José A. Andrades
3
,
José Becerra
3,5
,
Josep Samitier
3,2
1
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST)
,
2
Department of Engineering Electronics
,
University of Barcelona (UB)
,
3
Networking Biomedical Research Center (CIBER)
,
4
Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry
,
Universidad de Málaga (UMA)
,
5
Andalusian Centre for Nanomedicine and Biotechnology-BIONAND
,
6
Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas
,
Universidade da Coruña (UDC)
,
7
Institució Catalana de Recerca i Estudis Avançats (ICREA)
,
8
Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology
,
Universidad de Málaga (UMA)
Chapters
00:00
Title
01:12
Production of Nanopatterned Growth Substrates
05:59
Local RGD Surface Density Characterization with AFM
07:49
Cell Culture and Induction of Chondrogenesis
08:49
Cell Fixation and Immunostaining
10:18
Cell Imaging and Data Analysis
12:28
Representative Results
13:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了一种获得基于状的不均匀 nanopatterns 的方法, 该法允许对局部精氨酸-甘氨酸 (RGD) 的表面密度进行纳米控制, 并将其用于细胞黏附和软骨分化的研究。
Tags
Dendrimer
Nanopattern
Surface Adhesiveness
Chondrogenic Differentiation
RGD Peptide
PLLA
Spin Coating
UV Sterilization
Cell Adhesion
Microscopy
Immunostaining
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