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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Chapters
- 00:04Title
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
Nós relatamos um gancho de cabelo pequeno RNA (shRNA) e a próxima geração baseada no sequenciamento protocolo identificando os reguladores da inativação do cromossomo x em uma linha de celular murino com luciferase de vaga-lume e genes de resistência de hptII fundiram para o metil CpG ligação proteína 2 ( MeCP2) Gene no cromossomo X inativo.
