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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Chapters
- 00:04Title
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
Vi rapporterar en liten hårnål RNA (shRNA) och nästa generations sekvensering-baserat protokoll för att identifiera regulatorer av x-kromosomen inaktivering i en murin cellinje med firefly luciferas och hygromycin antibiotikaresistensgener smält till metyl CpG bindande protein 2) MeCP2) gen på inaktiva X-kromosomen.
