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Journal
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Neuroscience
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血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis
Please note that all translations are automatically generated.
Click here for the English version.
血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化
DOI:
10.3791/56407-v
•
10:30 min
•
November 16, 2017
•
Roberto Villaseñor
,
Ludovic Collin
1
Roche Pharma Research and Early Development (pRED), Neuroimmunology
,
Roche Innovation Center Basel
Chapters
00:05
Title
00:53
Generation of Brain Free-floating Sections
02:19
Cell or Organelle Labeling by Immunofluorescence Staining
04:27
High-resolution Confocal Imaging of the Blood-brain Barrier
06:21
Image Processing and 3D Reconstruction of the Neurovascular Unit
09:04
Results: Three-dimensional Rendering of Capillaries and Intracellular Vesicles at the Blood-brain Barrier
09:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个 microscopy-based 协议的高分辨率成像和三维重建的小鼠神经血管单位和血脑屏障使用脑漂浮部分。这种方法可用于血脑屏障内细胞器的可视化、分析和量化。
Tags
Blood-brain Barrier
Confocal Microscopy
3D Reconstruction
Transcytosis
Immunofluorescence
Free-floating Sections
Endothelial Cells
Neurovascular Unit
Subcellular Organelles
Quantification
Image Analysis
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