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Megakaryocyte differentiering og trombocyttal dannelse fra menneskelige ledning blod-afledte CD34+ celler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Megakaryocyte differentiering og trombocyttal dannelse fra menneskelige ledning blod-afledte CD34+ celler

DOI:
10.3791/56420-v

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09:46 min

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December 27, 2017

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, , ,
1Haematology Research Unit, St George and Sutherland Clinical School,University of New South Wales, 2Haematology Department,St George and Sutherland Hospitals

Chapters

  • 00:05Title
  • 01:06CD34+ Cord Blood Cell Isolation
  • 04:30Megakaryocyte Differentiation
  • 05:56Proplatelet Counting and Platelet Enumeration and Activation
  • 07:24Results: Representative Megakaryocyte Differentiation and Platelet Formation
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

En meget ren population af megakaryocytes kan fås fra ledningen blod-afledte CD34+ celler. En metode til CD34+ celle isolation og megakaryocyte differentiering er beskrevet her.

Tags

Megakaryocyte DifferentiationPlatelet FormationCord BloodCD34+ CellsDensity Gradient CentrifugationCD34 Magnetic BeadsMegakaryopoiesisLymphocytesErythrocytesMacrophages

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