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Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine
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JoVE Journal Neuroscience
Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine

Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine

DOI:
10.3791/56434-v

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09:00 min

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October 10, 2017

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, , , , ,
1Departments of Anesthesiology and Oncology,Mayo Clinic, Translational Science Track, Mayo Graduate School, 2Department of Radiology (Section of Interventional Pain Management),Mayo Clinic, 3Department of Neurologic Surgery,Mayo Clinic, 4Department of Orthopedic Surgery,Mayo Clinic

Chapters

  • 00:05Title
  • 01:02Preparing the Surgical Site on the Swine
  • 01:59Dissecting the Vertebrae
  • 04:07Single-level Laminotomy and Dissection of the DRG
  • 06:12Injecting the DRG
  • 07:54Results: Evaluating an Injection
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for laminotomy in swine that provides access to lumbar dorsal root ganglia (DRG) for intraganglionic injection. Injection progress is monitored intraoperatively and histologically confirmed up to 21 days after surgery. This protocol could be used for future preclinical studies involving DRG injection.

Tags

Keywords: LaminotomyLumbarDorsal Root GanglionInjectionSwineSurgical AccessIntraganglionic InjectionConvection-enhanced DeliveryNeuroscienceTherapeutic EfficacyDelivery EfficiencyAnesthesiaSurgical PreparationMidline IncisionHemostasisMonopolar ElectrosurgeryThoracal Lumbar FasciaSupraspinous LigamentSpinous ProcessesParasagittal Incision

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