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Multipleks cytokin profilering av stimulert musen Splenocytes bruker en Cytometric Bead-baserte immunanalyse plattform
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
DOI:

11:00 min

November 09, 2017

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Chapters

  • 00:05Title
  • 00:58Reagent and Standard Preparation
  • 02:57Assay Procedure
  • 06:23Flow Cytometer Set-up
  • 09:24Results: Quantification of Cytokines Produced by Mouse Splenocytes Cultured Under Stimulating Conditions
  • 10:15Conclusion

Summary

Automatic Translation

Denne protokollen beskriver kvantifisering av flere cytokin mål samtidig i vev kultur supernatants fra stimulert musen splenocytes multiplex perle basert immunanalyse plattform og en flyt cytometer.

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