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Høy gjennomstrømming parallelle sekvensering å måle Fitness Leptospira interrogans Transposon innsetting mutanter under Golden Syrian Hamster infeksjon
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1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine,David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology,David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics,University of California Los Angeles
Chapters
- 00:05Title
- 01:17Creation of the Transposon Mutant Library: Transfer of the Transposon into Leptospira spp. by Conjugation
- 03:56Animal Experiment
- 04:57Making the Genomic Library
- 09:24Results: Challenged Hamsters
- 10:59Conclusion
Her beskriver vi en teknikk som kombinerer transposon mutagenese med høy gjennomstrømming sekvensering å identifisere og telle transposon leptospiral mutanter i vev etter en utfordring hamstere. Denne protokollen kan brukes til skjermen mutanter for overlevelse og spredning i dyr og kan også bli brukt i vitro studier.
