Journal
/
Cancer Research
/
Evaluating In Vitro DNA Damage Using Comet Assay
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Evaluating In Vitro DNA Damage Using Comet Assay

Evaluating In Vitro DNA Damage Using Comet Assay

DOI:
10.3791/56450-v

•

10:43 min

•

October 11, 2017

•

, ,
1Neuro-Oncology Branch, Center for Cancer Research,National Cancer Institute, 2Basic Medical Science Department,Zunyi Medical College-Zhuhai Campus

Chapters

  • 00:05Title
  • 00:46Preparing Comet Slides
  • 03:17Single Cell Electrophoresis
  • 05:43Staining Comet Slides
  • 06:25Image Acquisition and Analysis
  • 08:35Results: Combining Olaparib with Temozolomide Enhances DNA damage in Glioma Cells
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

The comet assay is an efficient method to detect DNA damage including single and double-stranded DNA breaks. We describe alkaline and neutral comet assays to measure DNA damage in cancer cells to evaluate the therapeutic effect of chemotherapy.

Tags

Keywords: Comet AssayDNA DamageGenotoxic AgentsCancer CellsSingle Cell ElectrophoresisAlkaline Comet AssayAgaroseCell CultureGlioma CellsChemotherapyLysis SolutionDNA UnwindingElectrophoresis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56450?article_type=v

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
check_url/56450?article_type=v

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
check_url/56450?article_type=v

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
check_url/56450?article_type=v

In Vitro Assay to Study Tumor-macrophage Interaction
check_url/56450?article_type=v

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
check_url/56450?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
check_url/56450?article_type=v

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
check_url/56450?article_type=v

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
check_url/56450?article_type=v

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
check_url/56450?article_type=v

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

Read Article