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Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections
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Bioengineering
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JoVE Journal Bioengineering
Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections

Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections

DOI:
10.3791/56492-v

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12:21 min

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October 31, 2017

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, ,
1Department of Chemistry,University of Louisville, 2Department of Pharmacology and Toxicology,University of Louisville, 3Center for Predictive Medicine,University of Louisville, 4Department of Microbiology and Immunology,University of Louisville, 5Department of Bioengineering,University of Louisville

Chapters

  • 00:05Title
  • 01:08Preparation and Fabrication of the Electrospun Fiber Scaffold
  • 03:22Surface-modification of Fibers with GRFT
  • 06:35Extraction of GRFT from Surface-modified Fibers and Measuring GRFT Desorption from Fibers
  • 08:28Results: Characterization of Griffithsin-modified Fiber Scaffolds
  • 10:56Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes the procedure to fabricate and characterize Griffithsin-modified poly(lactic-co-glycolic acid) electrospun fibers that demonstrate potent adhesive and antiviral activity against human immunodeficiency virus type 1 infection in vitro. Methods used to synthesize, surface-modify, and characterize the resulting morphology, conjugation, and desorption of Griffithsin from surface-modified fibers are described.

Tags

Keywords: FabricationCharacterizationGriffithsinFiber ScaffoldsSexually Transmitted InfectionsPolylactic AcidCo-glycolic AcidElectrospun FibersAntiviral ProteinDrug DeliveryHIV-1ProphylacticTherapeuticPLGAHFIPElectrospinningSyringe PumpPBSMESEDCNHS

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