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Fremstilling og karakterisering af Griffithsin-ændret Fiber stilladser til forebyggelse af seksuelt overførte infektioner
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1Department of Chemistry,University of Louisville, 2Department of Pharmacology and Toxicology,University of Louisville, 3Center for Predictive Medicine,University of Louisville, 4Department of Microbiology and Immunology,University of Louisville, 5Department of Bioengineering,University of Louisville
Chapters
- 00:05Title
- 01:08Preparation and Fabrication of the Electrospun Fiber Scaffold
- 03:22Surface-modification of Fibers with GRFT
- 06:35Extraction of GRFT from Surface-modified Fibers and Measuring GRFT Desorption from Fibers
- 08:28Results: Characterization of Griffithsin-modified Fiber Scaffolds
- 10:56Conclusion
Dette manuskript beskriver proceduren, at fabrikere og karakterisere Griffithsin modificerede poly (mælkesyre-co-glycolic acid) electrospun fibre, der demonstrerer potent klæbende og antiviral aktivitet mod human immundefekt virus type 1-infektion i vitro. Metoder, der anvendes til at syntetisere, overflade-redigere, karakteriserer den resulterende morfologi, konjugering, og desorption for Griffithsin fra overfladen-modificerede fibre er beskrevet.
