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Neuroscience
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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞
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Neuroscience
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JoVE Journal
Neuroscience
Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞
DOI:
10.3791/56538-v
•
12:34 min
•
December 06, 2017
•
Asa S. Hidmark
,
Peter P. Nawroth
2,3
,
Thomas Fleming
3
1
Department of Medicine I and Clinical Chemistry
,
University Hospital of Heidelberg
,
2
Institute for Diabetes and Cancer IDC Helmholtz Center Munich
,
Germany & Joint Heidelberg-IDC Translational Diabetes Program
,
3
German Center for Diabetes Research (DZD)
Chapters
00:05
Title
01:28
Dissection of Sciatic Nerve and Dorsal Root Ganglion
04:02
Digestion of Sciatic Nerve and Dorsal Root Ganglion
06:36
Setup and Flow Cytometry and Running of Samples
10:00
Results: Flow Cytometry Analysis of Surface Expressed Markers on Singlets from the Sciatic Nerve and Dorsal Root Ganglion
11:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
由于组织的缺乏, 小鼠坐骨神经内细胞含量的定量分析是困难的。本协议描述了一种组织消化和制备的方法, 它提供了足够的细胞, 用于对单个小鼠神经的免疫细胞进行流式细胞仪分析。
Tags
Keywords: Flow Cytometry
Immune Cells
Sciatic Nerve
Dorsal Root Ganglion
Neuropathy
Neuroinflammation
Mouse
Dissection
Cell Isolation
Digestion
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