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Bevæger sig opad: En enkel og fleksibel In Vitro tredimensionale Invasion Assay protokol
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
DOI:

06:14 min

March 12, 2018

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Chapters

  • 00:05Title
  • 01:11Culture Plate, Collagen Gel Preparation, and Cell Imaging
  • 03:33Results: Representative Inverted Vertical Invasion Assay Analyses
  • 04:33Conclusion

Summary

Automatic Translation

Denne protokol beskriver en omvendt lodret invasion assay, der kunne bruges til at kvantificere migration og invasion kapaciteter af celler i en tre-dimensionelle indstilling samtidig bevare celle mikromiljø. Denne analyse er egnet for sjældne og/eller følsomme celler.

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