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Multipleksede immunofluorescens analyse og kvantificering af Intratumoral PD-1+ Tim-3+ CD8+ T celler
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1PARCC-INSERM U970,Université Paris Descartes, 2INSERM U970,Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology,Hopital Européen Georges Pompidou, 5Department of Medical Urology,Hopital Européen Georges Pompidou, 6Department of Pathology,Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology,Hopital Européen Georges Pompidou
Chapters
- 00:04Title
- 00:59Immunostaining
- 02:56Slide Scanning
- 04:30Image Analysis
- 07:16Results: Representative Intratumoral PD-1+Tim-3+CD8+ T cell Quantification and Analysis
- 08:51Conclusion
At studere tumor mikromiljø kan identificere prognostiske eller intelligent biomarkører for klinisk respons på immunterapi. Præsenteres her, er en nyskabende metode baseret på i situ fluorescens multispektrale imaging for at analysere og regne automatisk forskellige delpopulationer af CD8+ T celler. Denne reproducerbare og pålidelige teknik egner sig til stor kohorte analyser.
