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Chapters
- 00:05Title
- 00:52Recombinatorial Cloning to Generate Ready-for-injection Clones
- 02:23Hydrodynamic Tail Vein Injection
- 04:22Induction of Transfected CreER with Tamoxifen
- 05:33Induction of Tetracycline-dependent Gene or shRNA Expression
- 06:35Preparation of Mouse Liver for Analysis by Immunostaining
- 07:30Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI
- 08:47Conclusion
Etter halen blodåre injeksjon av transposon-baserte integrering vektorer gjør stabil transfection murine hepatocytes i vivo. Her presenterer vi en praktisk protokoll for transfection systemer som gjør det muliggjør langsiktige grunnleggende uttrykk for en enkelt transgene eller kombinert konstituerende og doxycycline-induserbart uttrykk av transgene eller miR-shRNA i leveren.
