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In Vitro SUMOylation analysen å studere SUMO E3 Ligase aktivitet
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JoVE Journal Biology
In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

In Vitro SUMOylation analysen å studere SUMO E3 Ligase aktivitet

DOI:
10.3791/56629-v

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09:45 min

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January 29, 2018

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, , ,
1Institute of Microbiology and Immunology,National Yang-Ming University, 2UC Davis Cancer Center,University of California, Davis, 3Department of Biochemistry and Molecular Medicine,University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology,Taipei Medical University, 5Division of Molecular and Genomic Medicine,National Health Research Institutes, 6Center for Infectious Disease and Cancer Research,Kaohsiung Medical University

Chapters

  • 00:05Title
  • 00:39Purification of K-bZIP
  • 03:26SUMOylation Assay
  • 07:15Results: Use of In Vitro SUMOylation Assay to Study the Role of SUMO E3 Ligase in p53 SUMOylation Using Optimal Ubc9 Enzyme Concentration
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

I motsetning til ubiquitin ligases, har noen E3 SUMO ligases blitt identifisert. Dette endret i vitro SUMOylation protokollen er kjøpedyktig identifisere romanen SUMO E3 ligases ved i vitro rekonstituering analysen.

Tags

Keywords: In Vitro SUMOylationSUMO E3 Ligase ActivitySUMO E3 Ligase IdentificationSUMO Isoform SpecificityProtein PurificationMagnetic Bead PulldownSDS-PAGEProtein QuantificationImage J

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