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组蛋白3赖氨酸 79 Dimethylation 标记沉淀后神经祖细胞的分离培养

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Neuroscience

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JoVE Journal Neuroscience

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

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组蛋白3赖氨酸 79 Dimethylation 标记沉淀后神经祖细胞的分离培养

DOI:

10.3791/56631-v

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10:09 min

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January 26, 2018

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Patrick Bovio², Deborah Roidl, Stefanie Heidrich, Tanja Vogel, Henriette Franz

¹Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine,University of Freiburg, ²Faculty of Biology,University of Freiburg

Chapters

00:05Title
00:49Isolation of Cerebellar Granular Neuron Progenitors (CGNP)
03:59Cell Fixation and Chromatin Shearing
05:53Preclearing
06:47Preparation of Antibody-Coupled Beads
07:37Chromatin Immunoprecipitation
08:51Results: H3K79me2 ChIP-qPCR and ChIP-Seq Analysis
09:41Conclusion

Summary

Automatic Translation

我们提出一种有效和可重复的方法, 分离和培养胚胎和产后脑组织中的神经祖细胞染色质沉淀 (芯片) 的组蛋白3赖氨酸 79 dimethylation (H3K79me2)-一个组蛋白标记位于组蛋白3的球状域。

Tags

Keywords: Neural Progenitors Chromatin Immunoprecipitation Histone 3 Lysine 79 Dimethylation Cerebral Cortex Cerebellum Brain Development Epigenetic Modifications In Vivo Histone Modifications Cell Culture Cerebellar Cells Astrocytes Poly-D-lysine

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