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宫内
电穿孔诱导小鼠大脑皮质中氧合酶的重组
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through
In Utero
Electroporation
Please note that all translations are automatically generated.
Click here for the English version.
宫内
电穿孔诱导小鼠大脑皮质中氧合酶的重组
DOI:
10.3791/56675-v
•
10:29 min
•
November 17, 2017
•
Katherine M. Bland*
1
,
Zachary O. Casey*
1
,
Christopher J. Handwerk*
1
,
Z. Logan Holley
,
George S. Vidal
1
Department of Biology
,
James Madison University
Chapters
00:05
Title
01:05
Preparation of Pulled Pipettes
02:29
In Utero
Electroporation
06:54
Brain Removal for Histological Preparation
08:06
Results: Expression of GFP After Electroporation
09:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在稀疏的细胞群中, 可以通过诱导功能的丧失或增益来研究脑内基因的细胞自主功能。在这里, 我们描述
在子宫内
电穿孔, 以提供重组到稀疏的人群发育皮层神经元与 floxed 基因, 导致功能丧失
在体内
。
Tags
Cre-lox Recombination
Mouse Cerebral Cortex
In Utero Electroporation
Genetic Mosaic
Neurobiology
Layer 2-3 Cortical Neurons
Pipette Preparation
Embryonic Day 15.5
Anesthesia
Mouse Surgery
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