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宫内电穿孔诱导小鼠大脑皮质中氧合酶的重组
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Neuroscience
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JoVE Journal Neuroscience
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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宫内电穿孔诱导小鼠大脑皮质中氧合酶的重组

DOI:
10.3791/56675-v

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10:29 min

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November 17, 2017

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, , , ,
1Department of Biology,James Madison University

Chapters

  • 00:05Title
  • 01:05Preparation of Pulled Pipettes
  • 02:29In Utero Electroporation
  • 06:54Brain Removal for Histological Preparation
  • 08:06Results: Expression of GFP After Electroporation
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

在稀疏的细胞群中, 可以通过诱导功能的丧失或增益来研究脑内基因的细胞自主功能。在这里, 我们描述在子宫内电穿孔, 以提供重组到稀疏的人群发育皮层神经元与 floxed 基因, 导致功能丧失在体内。

Tags

Cre-lox RecombinationMouse Cerebral CortexIn Utero ElectroporationGenetic MosaicNeurobiologyLayer 2-3 Cortical NeuronsPipette PreparationEmbryonic Day 15.5AnesthesiaMouse Surgery

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