Journal
/
Immunology and Infection
/
Visualisere intracellulære SNARE handel af fluorescens levetid Imaging mikroskopi
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualisere intracellulære SNARE handel af fluorescens levetid Imaging mikroskopi

DOI:
10.3791/56745-v

•

08:55 min

•

December 29, 2017

•

, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Chapters

  • 00:05Title
  • 00:37Preparation of the Microscopy Samples
  • 01:49Recording of the FLIM Data
  • 03:34Conversion of the Photon Recordings to FLIM Images
  • 04:19Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
  • 06:03Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en ny metode giver mulighed for kvantitative visualisering af komplekse dannelsen af SNARE proteiner, baseret på Förster resonans energioverførsel og fluorescens levetid imaging mikroskopi.

Tags

SNAREFLIMMembrane TraffickingHeLa CellsSyntaxin-4VAMP3Syntaxin-3MCitrineMCherryFluorescence Lifetime Imaging MicroscopyConfocal MicroscopyInstrument Response Function

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Single Cell Målinger af vacuolær Brud forårsaget af Intracellulære patogener

Opdagelsen af ​​nye intracellulære patogener af Amoebal cokultur og Amoebal Enrichment Approaches

Analyse af<em> Yersinia enterocolitica</em> Effector Translokation i værtsceller Brug Beta-lactamase Effector Fusions

Måling fagosom pH ved Ratiometrisk fluorescensmikroskopi

Ikke-invasiv<em> In vivo</em> Fluorescens optisk billeddannelse af inflammatorisk MMP Aktivitet Brug et aktiverbart Fluorescent Imaging Agent

Muse lymfocyt Mærkning af<sup> 64</sup> Cu-antistofreceptor Målretning for<em> In vivo</em> Cell Trafficking af PET / CT

Visualisere makrofag ekstracellulære fælder ved hjælp af konfokalmikroskopi

Integrere Imaging flowcytometri og transkriptom profilering for at vurdere ændrede Endocytic CD1d handel

Read Article