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The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
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JoVE Journal Cancer Research
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

DOI:
10.3791/56761-v

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07:39 min

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June 16, 2018

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, , , , , ,
1The Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University, 3Trauma Office,Children’s National Health System

Chapters

  • 00:04Title
  • 00:46Tumor Cell Recovery
  • 02:01Fluorescent Labeling and Lung Colonization
  • 04:17Confocal Microscopic Imaging
  • 05:20Results: Representative Lung Colonization Analyses
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.

Tags

Lung ColonizationCirculating Tumor CellsCancer MetastasisCell VisualizationCell CultureTrypsin-EDTACell SuspensionCentrifugationCFSETail Vein InjectionC57BL/6 Mouse

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