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Un Western Blot protocolo para una pequeña cantidad de células madre hematopoyéticas
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1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Chapters
- 00:04Title
- 00:48Sample Preparation
- 04:20Electrophoresis, Transfer, and Block
- 05:30Antibody Labeling and Detection
- 06:09Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
- 07:56Conclusion
Un estándar de Western Blot protocolo fue optimizado para el análisis de tan sólo 500 madre hematopoyética o células progenitoras. Optimización implica la cuidadosa manipulación de la muestra de células, limitando las transferencias entre los tubos y directamente lisis de las células en Tampón Laemmli.
