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Overflade teknik af pancreas Holme med en Heparinized StarPEG Nanocoating
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Overflade teknik af pancreas Holme med en Heparinized StarPEG Nanocoating

DOI:
10.3791/56879-v

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05:35 min

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June 23, 2018

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, , ,
1Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering,Chinese Academy of Medical Science & Peking Union Medical College

Chapters

  • 00:04Title
  • 00:52Fabrication of Heparin-incorporated StarPEG Nanocoating
  • 02:15Mouse Islet Surface Engineering with Heparin-PEG Nanocoating
  • 03:40Results: Characterization of Heparin-PEG Coated Mouse Islets
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol har til formål at opnå overflade teknik af pancreas Holme ved hjælp af heparin-indarbejdet starPEG nanocoating via pseudo-bioorthogonal kemi mellem N- hydroxysuccinimide grupper af nanocoating og amine grupper af holmen cellens membran.

Tags

Pancreatic IsletSurface EngineeringHeparinized StarPEG NanocoatingIslet TransplantationCell-based TherapyImmuno-rejectionRevascularizationSurvivalGraft FunctionTherapeutic EfficacyCell RetentionCell SurvivalHeparinNHSEDCStarPEGMouse IsletsPBSRPMI 1640FBS

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