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Genomet hele RNAi Screening å identifisere vert faktorer som modulerer kan viruset terapi
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1Children’s Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology,University of Ottawa, 3Department of Pediatrics,University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary
Chapters
- 00:05Title
- 00:45Preparation for Reverse Transcription
- 01:57Reverse Transfection
- 04:32Infection
- 05:52Fixing and Staining Cells
- 06:39Results: Genome-wide RNAi Screen to Identify Host Targets that Enhance Oncolytic Virus Therapy
- 08:13Conclusion
Her beskriver vi en protokoll for å ansette høy gjennomstrømming RNAi screening å avdekke vert mål som kan manipuleres til å forbedre kan viruset terapi, spesielt rhabodvirus og vaccinia virus terapi, men det kan være lett tilpasses andre kan virus plattformer eller for å oppdage vert gener som modulerer virus replikering generelt.
