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细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用

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Developmental Biology

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JoVE Journal Developmental Biology

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用

DOI:

10.3791/56919-v

•

05:48 min

•

January 02, 2018

•

Ashutosh Pandey, Hamed Jafar-Nejad²

¹Department of Molecular and Human Genetics,Baylor College of Medicine, ²Program in Developmental Biology,Baylor College of Medicine

Chapters

00:05Title
00:38Assessment of Binding Between Notch Receptor and Trans-ligands
01:47Aggregation Between Signal-sending and Signal-receiving Cells
03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
04:15Results: Faithful Analysis of Notch Signaling
05:30Conclusion

Summary

Automatic Translation

复杂的在体内系统, 使得很难区分的激活和抑制的缺口受体的跨-和cis配体, 分别。在这里, 我们提出了一个基于体外细胞聚合的方法, 用于定性和半定量评价的结合的果蝇缺口, 以跨配基与顺式配基。

Tags

Keywords: Cell Aggregation Assay Notch Receptor Trans-ligands Cis-ligands Drosophila S2 Cells Ligand Binding Cell Signaling Aggregation Quantification Microscopy Hemacytometer

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